Extracellular Vesicles: Fundamentals of Biology and Analysis
Learn the basics of extracellular vesicles, from biogenesis to isolation and characterization techniques.
Learn the basics of extracellular vesicles, from biogenesis to isolation and characterization techniques.
This comprehensive course introduces the fundamentals of extracellular vesicles (EVs), covering their biology, isolation, and analysis. Learn about EV types, biogenesis, cargo, and functions. Explore techniques for sample collection, isolation, and characterization. Gain practical knowledge for EV research and applications in biomedicine.
4.8
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What you'll learn
Discuss the nomenclature and subgroups of extracellular vesicles
Describe the RNA, protein, and lipid content of extracellular vesicles
Understand the basic concepts of common isolation and characterization techniques for EVs
Evaluate the benefits and limitations of various EV isolation and characterization methods
Explain the considerations for collecting and isolating EVs from different body fluids
Describe the release and uptake mechanisms of extracellular vesicles
Skills you'll gain
This course includes:
467 Minutes PreRecorded video
5 assignments
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There are 5 modules in this course
This course provides a comprehensive introduction to the field of extracellular vesicles (EVs), covering fundamental concepts and advanced analytical techniques. Participants will explore the history, nomenclature, and biological significance of EVs across various organisms. The curriculum delves into EV biogenesis, cargo composition (RNA, proteins, lipids), and cellular uptake mechanisms. Learners will gain practical knowledge on sample collection and processing from diverse sources, including cell culture media and body fluids. The course covers a range of isolation methods, such as ultracentrifugation, density gradients, and size exclusion chromatography. Additionally, students will learn about cutting-edge characterization techniques, including electron microscopy, flow cytometry, and nanoparticle tracking analysis. Through a combination of video lectures and assessments, this course equips researchers and professionals with the essential knowledge and skills for EV research and its applications in biomedicine.
Introduction to the Course and the Field of Extracellular Vesicles
Module 1 · 1 Hours to complete
Biogenesis, Cargo and Uptake of Extracellular Vesicles
Module 2 · 2 Hours to complete
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles
Module 3 · 1 Hours to complete
Methods for Isolating Extracellular Vesicles
Module 4 · 1 Hours to complete
Techniques for Characterization and Quantification of Extracellular Vesicles
Module 5 · 2 Hours to complete
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Instructor
Expert in Extracellular Vesicles and Their Role in Disease
Cecilia Lässer is a researcher at the Krefting Research Centre at the University of Gothenburg, Sweden, specializing in the RNA and protein cargo of extracellular vesicles, particularly exosomes. Her work involves dissecting subpopulations of extracellular vesicles to understand their biogenesis, cargo loading, biological function, and uptake. She is particularly focused on how RNA and protein cargo changes in extracellular vesicles during chronic inflammation and in patients with malignant melanoma. Cecilia holds a Master's degree in Molecular Biology and earned her Ph.D. in Medicine from the University of Gothenburg in 2013, with her thesis titled “Exosomes - Characterisation of the RNA and Protein Cargo.” She has extensive experience working with extracellular vesicles from various body fluids and cell types and has been a member of the International Society for Extracellular Vesicles since its inception in 2011.
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4.8 course rating
343 ratings
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